Device
Part:BBa_K935005:Experience
Designed by: Twaddle Lab Group: iGEM12_IvyTech-South_Bend (2012-10-02)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
We transformed E.coli with a series of our arsenic responsive constructs. BBa_K935005 contains three ArsR genes under the control of the pArs promoter. We found it sensitivity to 6-fold less than that BBa_K935002, which contains only one ArsR gene under the control of pArs and essentially the same as that of BBa_K935004, which contains two ArsR genes. In addition the magnitude of the response was reduced 10-fold less at 100uM arsenate compared to that of BBa-935002.
User Reviews
UNIQ25be75a05a4f9d68-partinfo-00000000-QINU UNIQ25be75a05a4f9d68-partinfo-00000001-QINU